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Pharmcia原裝葡聚糖凝膠G-100Sephadex G-100

更新時間： 2023-07-24
產(chǎn)品型號： Pharmcia17-0060-01
簡單描述
Pharmcia原裝葡聚糖凝膠G-100Sephadex G-100
別名：葡聚糖凝膠G-100，
規(guī)格：分離：肽及球形蛋白質：4000～150000；葡聚糖(線性分子)：1000～100000
包裝：10克
100克
CAS號：9050-94-6
保存條件：RT

詳細介紹

Pharmcia原裝葡聚糖凝膠G-100Sephadex G-100

Matrix	Cross-linked dextran
Particle Size Wet	100 µm-310 µm
Partical Size Dry	40 µm-120 µm
Pressure/Flow Specification	Care must be taken to avoid compressing the gel. Do not increase the pressure beyond 0.096 bar.
Fractionation [Mr] Globular Proteins	4 x 10³-1.5 x 10⁵
Fractionation [Mr] Dextrans	1 x 10³-1 x 10⁵
Exclusion Limit	> 1.5 x 10⁵
Autoclavable	In wet form (pH 7.0) at 120°C for 30 min

Sephadex G-100, 100 g

Application note
Purification and renaturation of recombinant proteins produced in Escherichia coli as inclusion bodies Summary Expression of eukaryotic genes in the cytoplasm of Escherichia coli often leads to the production and accumulation of the recombinant protein (r-protein) to levels of total cell protein that are higher than when secreted, in some instances by as much as 25%. The r-proteins so produced are frequently in the form of insoluble aggregates, so-called inclusion bodies (IBs). The IBs contain reduced and denatured proteins lacking biological activity. Their sizes and densities are also distinct from most of the bacterial proteins, which facilitates their separation from intracellular E. coli proteins and cell debris by slow speed centrifugation. A general protocol for the extraction and purification of IBs from E. coli cells is shown. The purified IBs are usually dissolved in 6 M guanidine hydrochloride or 8 M urea at near neutral pH prior to re-naturation and purification of the r-protein. The most commonly used procedure for refolding of such de-natured r-proteins is slow dialysis, or dilution into a buffer of near neutral pH. This result in a significant dilution of the r-protein, typically to a few mg of r-protein per mL of solution, with the formation of precipitated proteins being often unavoidable. Alternative purification and/or refolding procedures using gel filtration, ion exchange or hydrophobic interaction chromatography (HIC) have been developed to circumvent such problems. These are outlined briefly in this Application Note. Close

Application note

Purification and renaturation of recombinant proteins produced in Escherichia coli as inclusion bodies Summary

Expression of eukaryotic genes in the cytoplasm of Escherichia coli often leads to the production and accumulation of the recombinant protein (r-protein) to levels of total cell protein that are higher than when secreted, in some instances by as much as 25%. The r-proteins so produced are frequently in the form of insoluble aggregates, so-called inclusion bodies (IBs). The IBs contain reduced and denatured proteins lacking biological activity. Their sizes and densities are also distinct from most of the bacterial proteins, which facilitates their separation from intracellular E. coli proteins and cell debris by slow speed centrifugation. A general protocol for the extraction and purification of IBs from E. coli cells is shown. The purified IBs are usually dissolved in 6 M guanidine hydrochloride or 8 M urea at near neutral pH prior to re-naturation and purification of the r-protein. The most commonly used procedure for refolding of such de-natured r-proteins is slow dialysis, or dilution into a buffer of near neutral pH. This result in a significant dilution of the r-protein, typically to a few mg of r-protein per mL of solution, with the formation of precipitated proteins being often unavoidable. Alternative purification and/or refolding procedures using gel filtration, ion exchange or hydrophobic interaction chromatography (HIC) have been developed to circumvent such problems. These are outlined briefly in this Application Note. Close

Handbook
Size Exclusion Chromatography: Principles and Methods Summary This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close

Handbook

Size Exclusion Chromatography: Principles and Methods Summary

This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close

Magazine
Upstream Downstream issue 2 Close

Material Safety Data Sheet
Sephadex™ G-100, 100 g Close
Sephadex™ G-100, 100 g Close

Pharmcia原裝葡聚糖凝膠G-100Sephadex G-100

17-0060-01	葡聚糖凝膠G-100	Sephadex G-100
17-0050-01	葡聚糖凝膠G-75	Sephadex G-75
17-0043-01	葡聚糖凝膠G-50	Sephadex G-50
17-0033-01	葡聚糖凝膠G-25[中]	Sephadex G-25
17-0020-01	葡聚糖凝膠G-15	Sephadex G-15
17-0010-01	葡聚糖凝膠G-10	Sephadex G-10
B-2261	熒光染糖[NO.33342]	bisBenzimide H 33342 trihydrochloride
17-0170-01	DEAE葡聚糖凝膠A-25	DEAE Sephadex A-25
17-0170-02	DEAE葡聚糖凝膠A-25	DEAE Sephadex A-25
17-0180-01	DEAE葡聚糖凝膠A-50	DEAE Sephadex A-50
17-0180-02	DEAE葡聚糖凝膠A-50	DEAE Sephadex A-50
G-9382	明膠	Gelatin
L-5125	N-十二烷基肌氨酸鈉	N-Lauroylsarcosine sodium salt
L-5125	N-十二烷基肌氨酸鈉	N-Lauroylsarcosine sodium salt
0719	N-十二烷基肌氨酸鈉	N-Lauroylsarcosine sodium salt
0719	N-十二烷基肌氨酸鈉	N-Lauroylsarcosine sodium salt
T-8768	3,3'5,5'-四甲基聯(lián)苯胺二鹽酸	TMB 2Hcl
T-8768	3,3'5,5'-四甲基聯(lián)苯胺二鹽酸	TMB 2Hcl
0759	3,3'5,5'-四甲基聯(lián)苯胺二鹽酸	TMB 2Hcl
0759	3,3'5,5'-四甲基聯(lián)苯胺二鹽酸	TMB 2Hcl
E-2129	埃文斯藍[伊文斯藍]	Evans Blue
E-2129	埃文斯藍[伊文斯藍]	Evans Blue
19555	埃文斯藍[伊文斯藍]	Evans Blue
19555	埃文斯藍[伊文斯藍]	Evans Blue
0335	蔗糖	Sucrose
21938	蔗糖	Sucrose
F-0127	D-果糖	D-(-)Fructose
0226	D-果糖	D-(-)Fructose
0528	結晶紫	crystal violet
0671	D-核糖	D-Ribose
J392	棉子糖	D-Raffinose
P3504	麗春紅S	Ponceau S
0860	麗春紅S	Ponceau S
C-2037	細胞色素C[牛心]	Cytochromes C(Bovine Heart)
C-7752	細胞色素C[馬心]	Cytochromes C(Horse Heart)
0322		EDTA
0322		EDTA
D-4551	N,N-二甲基甲酰胺	N,N-Dimethylformamide
0464	N,N-二甲基甲酰胺	N,N-Dimethylformamide
T-9250	胸腺嘧啶核苷	Thymidine
T-9250	胸腺嘧啶核苷	Thymidine
22305	胸腺嘧啶核苷	Thymidine
22305	胸腺嘧啶核苷	Thymidine
P-3250	木瓜蛋白酶	Papain from papaya latex
P-3250	木瓜蛋白酶	Papain from papaya latex
G-3632	硫酸慶大霉素	Gentamicin sulfate salt
G-3632	硫酸慶大霉素	Gentamicin sulfate salt
0304	硫酸慶大霉素	Gentamicin sulfate salt
0304	硫酸慶大霉素	Gentamicin sulfate salt
C-7698	[環(huán)已亞胺]	Cycloheximide
C-7698	[環(huán)已亞胺]	Cycloheximide
D-1756	地塞米松（氫化可的松）	Dexamethasone
D-1756	地塞米松（氫化可的松）	Dexamethasone
A-7506	維生素C[抗球血酸鈉]	L-Ascorbic acid[Vitamin C]
A-7506	維生素C[抗球血酸鈉]	L-Ascorbic acid[Vitamin C]
0764	維生素C[抗球血酸鈉]	L-Ascorbic acid[Vitamin C]
0764	維生素C[抗球血酸鈉]	L-Ascorbic acid[Vitamin C]
*******	維生素C[抗球血酸鈉]	L-Ascorbic acid[Vitamin C]
*******	氯化鋰	Lithium chloride
0416	氯化鋰	Lithium chloride
22178	纖維素酶	Cellulase from Aspergillus niger
********	纖維素酶	Cellulase from Aspergillus niger
M-8410	礦物油[石臘油]	Mineral oil
J217	礦物油[石臘油]	Mineral oil
C-7880	L-半胱氨酸鹽酸鹽	L-Cysteine hydrochloride monohydrate
C-7880	L-半胱氨酸鹽酸鹽	L-Cysteine hydrochloride monohydrate
0206	L-半胱氨酸鹽酸鹽	L-Cysteine hydrochloride monohydrate
0206	L-半胱氨酸鹽酸鹽	L-Cysteine hydrochloride monohydrate
E-4378	乙二醇-雙-(2-氨基乙基)四乙酸	Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid[EGTA]
0732	乙二醇-雙-(2-氨基乙基)四乙酸	Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid[EGTA]
T-8877	2,3,5-氯化三苯基四氮唑	2,3,5-Triphenyltetrazolium chloride[TTC]
0765	2,3,5-氯化三苯基四氮唑	2,3,5-Triphenyltetrazolium chloride[TTC]
B-0252	硼酸	Boric acid
0588	硼酸	Boric acid
P-3640	聚乙二醇 3350	PEG 3350
P-3640	聚乙二醇 3350	PEG 3350
817006	聚乙二醇 4000	PEG 4000
817006	聚乙二醇 4000	PEG 4000
807491	聚乙二醇 6000	PEG 6000
807491	聚乙二醇 6000	PEG 6000
0159	聚乙二醇 8000	PEG 8000
0159	聚乙二醇 8000	PEG 8000
81285	聚乙二醇 12000	PEG 12000
81285	聚乙二醇 12000	PEG 12000
818897	聚乙二醇 20000	PEG 20000
818897	聚乙二醇 20000	PEG 20000
K-3378	激動素[6-呋喃氨基嘌呤]	6-Furfuryl Aminopurine(Kinetin)[KT]
K-3378	激動素[6-呋喃氨基嘌呤]	6-Furfuryl Aminopurine(Kinetin)[KT]
17980	激動素[6-呋喃氨基嘌呤]	6-Furfuryl Aminopurine(Kinetin)[KT]
17980	激動素[6-呋喃氨基嘌呤]	6-Furfuryl Aminopurine(Kinetin)[KT]
N-3503	制霉菌素	Nystatin
N-3503	制霉菌素	Nystatin
19770	制霉菌素	Nystatin
19770	制霉菌素	Nystatin
D-5637	3,3'-二氨基聯(lián)苯胺	3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
D-5637	3,3'-二氨基聯(lián)苯胺	3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
0430	3,3'-二氨基聯(lián)苯胺	3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
0430	3,3'-二氨基聯(lián)苯胺	3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
I-2886	吲哚-3-乙酸	3-Indoleacetic acid[IAA]
I-2886	吲哚-3-乙酸	3-Indoleacetic acid[IAA]
17550	吲哚-3-乙酸	3-Indoleacetic acid[IAA]
17550	吲哚-3-乙酸	3-Indoleacetic acid[IAA]
A-5006	L-精氨酸	L-Arginine
A-5006	L-精氨酸	L-Arginine
11490	L-精氨酸	L-Arginine
11490	L-精氨酸	L-Arginine
M-9625	L-甲硫氨酸[L-蛋氨酸]	L-Methionine
M-9625	L-甲硫氨酸[L-蛋氨酸]	L-Methionine
18915	L-甲硫氨酸[L-蛋氨酸]	L-Methionine
18915	L-甲硫氨酸[L-蛋氨酸]	L-Methionine
G-8270	D-葡萄糖[無水]	D-(+)-Glucose
0188	D-葡萄糖[無水]	D-(+)-Glucose
F-9037	去離子甲酰胺	Formamide,Deionized
0606	去離子甲酰胺	Formamide,Deionized